› 4owz

X-ray diffraction
1.47Å resolution

Structure of ECP/H15A mutant.

Released:
DOI: 10.2210/pdb4owz/pdb
PDB entry 4owz coloured by chain, front view.
PDB entry 4owz coloured by chain, side view.
PDB entry 4owz coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Blanco JA, Salazar VA, Boix E, Moussaoui M

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146306 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eosinophil cationic protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 134 amino acids
Theoretical weight: 15.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12724 (Residues: 28-160; Coverage: 100%)
Gene names: ECP, RNASE3, RNS3
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:
Ligand CIT 4 x CIT
Ligand FE 2 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P4322
Unit cell:
a: 62.543Å b: 62.543Å c: 175.233Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 mentions without citation

Exploring the mechanisms of action of human secretory RNase 3 and RNase 7 against Candida albicans.
Salazar et al. (2016)
1 more