› 4oz1

X-ray diffraction
1.74Å resolution

Crystal structure of human CAPERalpha UHM bound to SF3b155 ULM5

Released:
DOI: 10.2210/pdb4oz1/pdb
PDB entry 4oz1 coloured by chain, front view.
PDB entry 4oz1 coloured by chain, side view.
PDB entry 4oz1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cancer-relevant splicing factor CAPERĪ± engages the essential splicing factor SF3b155 in a specific ternary complex.
Loerch S, Maucuer A, Manceau V, Green MR, Kielkopf CL
J Biol Chem 289 17325-37 (2014)
PMID: 24795046

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131044 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RNA-binding protein 39 Chains: A, B
Molecule details ›
Chains: A, B
Length: 115 amino acids
Theoretical weight: 12.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q14498 (Residues: 417-530; Coverage: 22%)
Gene names: HCC1, RBM39, RNPC2
Structure domains: Alpha-Beta Plaits
Splicing factor 3B subunit 1 Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.31 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O75533 (Residues: 333-342; Coverage: 1%)
Gene names: SAP155, SF3B1

Ligands and Environments

3 bound ligands:
Ligand K 1 x K
Ligand CL 1 x CL
Ligand LYS 1 x LYS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P212121
Unit cell:
a: 44.653Å b: 52.41Å c: 85.141Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.143 0.18
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

9 review citations

Altered RNA Processing in Cancer Pathogenesis and Therapy.
Obeng et al. (2019)
8 more

6 mentions without citation

Unmasking the U2AF homology motif family: a bona fide protein-protein interaction motif in disguise.
Loerch et al. (2016)
5 more