› 4p0c

X-ray diffraction
1.34Å resolution

Crystal Structure of NHERF2 PDZ1 Domain in Complex with LPA2

Released:
DOI: 10.2210/pdb4p0c/pdb
PDB entry 4p0c coloured by chain, front view.
PDB entry 4p0c coloured by chain, side view.
PDB entry 4p0c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into PDZ-mediated interaction of NHERF2 and LPA(2), a cellular event implicated in CFTR channel regulation.
Holcomb J, Jiang Y, Lu G, Trescott L, Brunzelle J, Sirinupong N, Li C, Naren AP, Yang Z
Biochem Biophys Res Commun 446 399-403 (2014)
PMID: 24613836

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172337 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+)/H(+) exchange regulatory cofactor NHE-RF2 Chain: A
Molecule details ›
Chain: A
Length: 88 amino acids
Theoretical weight: 9.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15599 (Residues: 9-90; Coverage: 24%)
Gene names: NHERF2, SLC9A3R2
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

2 bound ligands:
Ligand SCN 1 x SCN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 26.36Å b: 40.25Å c: 37.1Å
α: 90° β: 107.42° γ: 90°
R-values:
R R work R free
0.147 0.145 0.177
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Emerging Roles of Diacylglycerol-Sensitive TRPC4/5 Channels.
Mederos Y Schnitzler et al. (2018)
2 more