› 4p0t

X-ray diffraction
1.49Å resolution

Crystal structure of human centromere protein M

Released:
DOI: 10.2210/pdb4p0t/pdb
PDB entry 4p0t coloured by chain, front view.
PDB entry 4p0t coloured by chain, side view.
PDB entry 4p0t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The pseudo GTPase CENP-M drives human kinetochore assembly.
Basilico F, Maffini S, Weir JR, Prumbaum D, Rojas AM, Zimniak T, De Antoni A, Jeganathan S, Voss B, van Gerwen S, Krenn V, Massimiliano L, Valencia A, Vetter IR, Herzog F, Raunser S, Pasqualato S, Musacchio A
Elife 3 e02978 (2014)
PMID: 25006165

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192594 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Centromere protein M Chains: A, B
Molecule details ›
Chains: A, B
Length: 176 amino acids
Theoretical weight: 19.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NSP4 (Residues: 1-171; Coverage: 95%)
Gene names: C22orf18, CENPM, ICEN39, PANE1
Sequence domains: Centromere protein M (CENP-M)
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3
Unit cell:
a: 104.03Å b: 104.03Å c: 33.56Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.127 0.124 0.164
Expression system: Escherichia coli

Quick links

Citations

17 review citations

The molecular basis for centromere identity and function.
McKinley et al. (2016)
16 more

2 mentions without citation

The pseudo GTPase CENP-M drives human kinetochore assembly.
Basilico et al. (2014)
1 more