› 4p3c

X-ray diffraction
1.94Å resolution

MT1-MMP:Fab complex (Form I)

Released:
DOI: 10.2210/pdb4p3c/pdb
PDB entry 4p3c coloured by chain, front view.
PDB entry 4p3c coloured by chain, side view.
PDB entry 4p3c coloured by chain, top view.
Source organisms:
Primary publication:
Inhibition mechanism of membrane metalloprotease by an exosite-swiveling conformational antibody.
Udi Y, Grossman M, Solomonov I, Dym O, Rozenberg H, Moreno V, Cuniasse P, Dive V, Arroyo AG, Sagi I
Structure 23 104-115 (2015)
PMID: 25482542

Function and Biology Details

Reaction catalysed: 
Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229965 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Heavy Chain Fab fragment of antibody LEM-2/15 Chain: H
Molecule details ›
Chain: H
Length: 218 amino acids
Theoretical weight: 23.49 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Light Chain Fab fragment of antibody LEM-2/15 Chain: L
Molecule details ›
Chain: L
Length: 218 amino acids
Theoretical weight: 23.96 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Matrix metalloproteinase-14 Chain: M
Molecule details ›
Chain: M
Length: 13 amino acids
Theoretical weight: 1.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P50281 (Residues: 215-227; Coverage: 2%)
Gene name: MMP14

Ligands and Environments

5 bound ligands:
Ligand CL 1 x CL
Ligand EPE 1 x EPE
Ligand EDO 6 x EDO
Ligand ACT 3 x ACT
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Unit cell:
a: 52.314Å b: 80.975Å c: 95.568Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.152 0.2
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21

Quick links

Citations

13 review citations

The Rebirth of Matrix Metalloproteinase Inhibitors: Moving Beyond the Dogma.
Fields GB. (2019)
12 more