4p5t

X-ray diffraction
3.26Å resolution

14.C6 TCR complexed with MHC class II I-Ab/3K peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-213590 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
TRA protein Chains: A, E
Molecule details ›
Chains: A, E
Length: 206 amino acids
Theoretical weight: 23.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Human nkt tcr beta chain Chains: B, F
Molecule details ›
Chains: B, F
Length: 241 amino acids
Theoretical weight: 27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
H-2 class II histocompatibility antigen, A-B alpha chain Chains: C, G
Molecule details ›
Chains: C, G
Length: 182 amino acids
Theoretical weight: 20.6 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P14434 (Residues: 27-208; Coverage: 78%)
Gene name: H2-Aa
Sequence domains:
Structure domains:
H-2 class II histocompatibility antigen, A beta chain Chains: D, H
Molecule details ›
Chains: D, H
Length: 217 amino acids
Theoretical weight: 24.97 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P14483 (Residues: 24-218; Coverage: 80%)
Gene names: H2-Ab1, H2-iabeta
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 66.007Å b: 74.142Å c: 259.289Å
α: 90° β: 92° γ: 90°
R-values:
R R work R free
0.189 0.186 0.236
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda