› 4p65

X-ray diffraction
1.5Å resolution

Crystal structure of an cyclohexylalanine substituted insulin analog.

Released:
DOI: 10.2210/pdb4p65/pdb
PDB entry 4p65 coloured by chain, front view.
PDB entry 4p65 coloured by chain, side view.
PDB entry 4p65 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Aromatic anchor at an invariant hormone-receptor interface: function of insulin residue B24 with application to protein design.
Pandyarajan V, Smith BJ, Phillips NB, Whittaker L, Cox GP, Wickramasinghe N, Menting JG, Wan ZL, Whittaker J, Ismail-Beigi F, Lawrence MC, Weiss MA
J Biol Chem 289 34709-27 (2014)
PMID: 25305014

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134235 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 30 amino acids
Theoretical weight: 3.42 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

3 bound ligands:
Ligand IPH 6 x IPH
Ligand ZN 2 x ZN
Ligand CL 2 x CL
2 modified residues:
Ligand ALC 6 x ALC
Ligand ORN 6 x ORN

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 46.04Å b: 60.9Å c: 59.3Å
α: 90° β: 112.25° γ: 90°
R-values:
R R work R free
0.164 0.16 0.203
Expression system: Not provided

Quick links

Citations

3 review citations

A thing of beauty: Structure and function of insulin's "aromatic triplet".
Weiss et al. (2018)
2 more

1 mention without citation

Aromatic anchor at an invariant hormone-receptor interface: function of insulin residue B24 with application to protein design.
Pandyarajan et al. (2014)