› 4p6x

X-ray diffraction
2.5Å resolution

Crystal Structure of cortisol-bound glucocorticoid receptor ligand binding domain

Released:
DOI: 10.2210/pdb4p6x/pdb
PDB entry 4p6x coloured by chain, front view.
PDB entry 4p6x coloured by chain, side view.
PDB entry 4p6x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures and mechanism for the design of highly potent glucocorticoids.
He Y, Yi W, Suino-Powell K, Zhou XE, Tolbert WD, Tang X, Yang J, Yang H, Shi J, Hou L, Jiang H, Melcher K, Xu HE
Cell Res 24 713-26 (2014)
PMID: 24763108

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137445 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glucocorticoid receptor Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 255 amino acids
Theoretical weight: 29.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P04150 (Residues: 523-777; Coverage: 33%)
Gene names: GRL, NR3C1
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 2 Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 14 amino acids
Theoretical weight: 1.71 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15596 (Residues: 740-753; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2

Ligands and Environments

1 bound ligand:
Ligand HCY 6 x HCY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P61
Unit cell:
a: 220.824Å b: 220.824Å c: 74.198Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.248 0.246 0.276
Expression systems:
  • Escherichia coli K-12
  • Not provided

Quick links

Citations

7 review citations

Corticosteroids in ophthalmology: drug delivery innovations, pharmacology, clinical applications, and future perspectives.
Gaballa et al. (2021)
6 more

23 mentions without citation

Glucocorticoid receptor control of transcription: precision and plasticity via allostery.
Weikum et al. (2017)
22 more