› 4p9h

X-ray diffraction
3Å resolution

Crystal structure of 8ANC195 Fab in complex with gp120 of 93TH057 HIV-1 and soluble CD4 D1D2

Released:
DOI: 10.2210/pdb4p9h/pdb
PDB entry 4p9h coloured by chain, front view.
PDB entry 4p9h coloured by chain, side view.
PDB entry 4p9h coloured by chain, top view.
Source organisms:
Primary publication:
Antibody 8ANC195 reveals a site of broad vulnerability on the HIV-1 envelope spike.
Scharf L, Scheid JF, Lee JH, West AP, Chen C, Gao H, Gnanapragasam PN, Mares R, Seaman MS, Ward AB, Nussenzweig MC, Bjorkman PJ
Cell Rep 7 785-95 (2014)
PMID: 24767986

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-229768 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (9 distinct):
T-cell surface glycoprotein CD4 Chain: C
Molecule details ›
Chain: C
Length: 192 amino acids
Theoretical weight: 21.44 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P01730 (Residues: 26-207; Coverage: 42%)
Gene name: CD4
Sequence domains:
Structure domains: Immunoglobulins
Envelope glycoprotein gp160 Chain: G
Molecule details ›
Chain: G
Length: 361 amino acids
Theoretical weight: 40.27 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q0ED31 (Residues: 43-122; Coverage: 9%)
Gene name: env
Structure domains: Human immunodeficiency virus 1, Gp160, envelope glycoprotein
Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 244 amino acids
Theoretical weight: 26.13 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand EPE 1 x EPE
Ligand BEN 2 x BEN
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 66.529Å b: 132.492Å c: 142.769Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.235 0.272
Expression systems:
  • Spodoptera frugiperda
  • Homo sapiens

Quick links

Citations

35 review citations

Broadly Neutralizing Antibodies to HIV and Their Role in Vaccine Design.
Burton et al. (2016)
34 more

6 mentions without citation

Antibody 8ANC195 reveals a site of broad vulnerability on the HIV-1 envelope spike.
Scharf et al. (2014)
5 more