› 4pam

X-ray diffraction
2.1Å resolution

A conserved phenylalanine as relay between the 5 helix and the GDP binding region of heterotrimeric G protein

Released:
DOI: 10.2210/pdb4pam/pdb
PDB entry 4pam coloured by chain, front view.
PDB entry 4pam coloured by chain, side view.
PDB entry 4pam coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
A conserved phenylalanine as a relay between the α5 helix and the GDP binding region of heterotrimeric Gi protein α subunit.
Kaya AI, Lokits AD, Gilbert JA, Iverson TM, Meiler J, Hamm HE
J Biol Chem 289 24475-87 (2014)
PMID: 25037222

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.36 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10824 (Residues: 1-354; Coverage: 100%)
Gene names: Gnai-1, Gnai1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GDP 1 x GDP
Ligand SO3 1 x SO3
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: I4
Unit cell:
a: 121.126Å b: 121.126Å c: 68.186Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.188 0.218
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Structure and dynamics of GPCR signaling complexes.
Hilger et al. (2018)
7 more

2 mentions without citation

A conserved phenylalanine as a relay between the α5 helix and the GDP binding region of heterotrimeric Gi protein α subunit.
Kaya et al. (2014)
1 more