› 4pdc

X-ray diffraction
1.99Å resolution

Crystal structure of Cowpox virus CPXV018 (OMCP) bound to human NKG2D

Released:
DOI: 10.2210/pdb4pdc/pdb
PDB entry 4pdc coloured by chain, front view.
PDB entry 4pdc coloured by chain, side view.
PDB entry 4pdc coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of cowpox evasion of NKG2D immunosurveillance
Lazear E, Sun MM, Wang X, Geurs TL, Nelson CA, Campbell JA, Lippold D, Krupnick AS, Davis RS, Carayannopoulos LN, French AR, Fremont DH.
(2019)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150837 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NKG2-D type II integral membrane protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 123 amino acids
Theoretical weight: 14.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26718 (Residues: 93-215; Coverage: 57%)
Gene names: D12S2489E, KLRK1, NKG2D
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A
CPXV018 protein Chains: E, F
Molecule details ›
Chains: E, F
Length: 150 amino acids
Theoretical weight: 17.85 KDa
Source organism: Cowpox virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8QN43 (Residues: 20-168; Coverage: 87%)
Gene name: CPXV018 CDS
Structure domains: MHC class I-like antigen recognition-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P21
Unit cell:
a: 43.315Å b: 101.11Å c: 91.368Å
α: 90° β: 91.63° γ: 90°
R-values:
R R work R free
0.168 0.166 0.214
Expression system: Escherichia coli BL21(DE3)

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