› 4pig

X-ray diffraction
1.95Å resolution

Crystal structure of the ubiquitin K11S mutant

Released:
DOI: 10.2210/pdb4pig/pdb
PDB entry 4pig coloured by chain, front view.
PDB entry 4pig coloured by chain, side view.
PDB entry 4pig coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Enhancing ubiquitin crystallization through surface-entropy reduction.
Loll PJ, Xu P, Schmidt JT, Melideo SL
Acta Crystallogr F Struct Biol Commun 70 1434-42 (2014)
PMID: 25286958

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158846 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 76 amino acids
Theoretical weight: 8.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62987 (Residues: 1-76; Coverage: 59%)
Gene names: UBA52, UBCEP2
Sequence domains: Ubiquitin family

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand CL 8 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P212121
Unit cell:
a: 47.7Å b: 61.82Å c: 91.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.218
Expression system: Escherichia coli

Quick links

Citations

2 mentions without citation

Microarray Analysis of Gene Expression in the Uterine Endometrium during the Implantation Period in Pigs.
Kim et al. (2012)
1 more