› 4pjw

X-ray diffraction
2.85Å resolution

crystal structure of human Stromal Antigen 2 (SA2) in complex with Sister Chromatid Cohesion protein 1 (Scc1), with bound MES

Released:
DOI: 10.2210/pdb4pjw/pdb
PDB entry 4pjw coloured by chain, front view.
PDB entry 4pjw coloured by chain, side view.
PDB entry 4pjw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion.
Hara K, Zheng G, Qu Q, Liu H, Ouyang Z, Chen Z, Tomchick DR, Yu H
Nat Struct Mol Biol 21 864-70 (2014)
PMID: 25173175

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129912 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cohesin subunit SA-2 Chain: A
Molecule details ›
Chain: A
Length: 981 amino acids
Theoretical weight: 115.44 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q8N3U4 (Residues: 80-1060; Coverage: 80%)
Gene names: SA2, STAG2
Sequence domains:
64-kDa C-terminal product Chain: B
Molecule details ›
Chain: B
Length: 140 amino acids
Theoretical weight: 16.24 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O60216 (Residues: 281-420; Coverage: 22%)
Gene names: HR21, KIAA0078, NXP1, RAD21, SCC1

Ligands and Environments

1 bound ligand:
Ligand MES 1 x MES
1 modified residue:
Ligand MSE 43 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 78.733Å b: 108.045Å c: 180.835Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.225
Expression system: Trichoplusia ni

Quick links

Citations

23 review citations

SMC complexes: from DNA to chromosomes.
Uhlmann F. (2016)
22 more

4 mentions without citation

Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion.
Hara et al. (2014)
3 more