› 4pl6

X-ray diffraction
1.68Å resolution

Structure of the chromodomain of MRG2 in complex with H3K4me3

Released:
DOI: 10.2210/pdb4pl6/pdb
PDB entry 4pl6 coloured by chain, front view.
PDB entry 4pl6 coloured by chain, side view.
PDB entry 4pl6 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Regulation of arabidopsis flowering by the histone mark readers MRG1/2 via interaction with CONSTANS to modulate FT expression.
Bu Z, Yu Y, Li Z, Liu Y, Jiang W, Huang Y, Dong AW
PLoS Genet 10 e1004617 (2014)
PMID: 25211338

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157776 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein MRG2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 75 amino acids
Theoretical weight: 9.12 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4V3E2 (Residues: 51-123; Coverage: 22%)
Gene names: At1g02740, F22D16.25, MRG2
Sequence domains: RNA binding activity-knot of a chromodomain
Structure domains: SH3 type barrels.
Histone H3.3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 11 amino acids
Theoretical weight: 1.29 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
UniProt:
  • Canonical: P59169 (Residues: 2-12; Coverage: 8%)
Gene names: At4g40030, At4g40040, At5g10980, HTR4, HTR5, HTR8, T30N20_250, T5J17.200, T5J17.210, T5K6.6

Ligands and Environments

No bound ligands
1 modified residue:
Ligand M3L 2 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P65
Unit cell:
a: 54.231Å b: 54.231Å c: 127.922Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.19 0.214
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

15 review citations

Tug of war: adding and removing histone lysine methylation in Arabidopsis.
Xiao et al. (2016)
14 more

4 mentions without citation

Regulation of arabidopsis flowering by the histone mark readers MRG1/2 via interaction with CONSTANS to modulate FT expression.
Bu et al. (2014)
3 more