› 4po2

X-ray diffraction
2Å resolution

Crystal Structure of the Stress-Inducible Human Heat Shock Protein HSP70 Substrate-Binding Domain in Complex with Peptide Substrate

Released:
DOI: 10.2210/pdb4po2/pdb
PDB entry 4po2 coloured by chain, front view.
PDB entry 4po2 coloured by chain, side view.
PDB entry 4po2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the stress-inducible human heat shock protein 70 substrate-binding domain in complex with peptide substrate.
Zhang P, Leu JI, Murphy ME, George DL, Marmorstein R
PLoS One 9 e103518 (2014)
PMID: 25058147

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-165039 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Heat shock 70 kDa protein 1A/1B Chains: A, B
Molecule details ›
Chains: A, B
Length: 235 amino acids
Theoretical weight: 26.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains:
HSP70 substrate peptide Chains: C, D
Molecule details ›
Chains: C, D
Length: 7 amino acids
Theoretical weight: 787 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 100.293Å b: 85.282Å c: 72.199Å
α: 90° β: 126.97° γ: 90°
R-values:
R R work R free
0.177 0.175 0.222
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

15 review citations

Present Yourself! By MHC Class I and MHC Class II Molecules.
Rock et al. (2016)
14 more

29 mentions without citation

Hsp70 in Redox Homeostasis.
Zhang et al. (2022)
28 more