› 4po7

X-ray diffraction
2.66Å resolution

Structure of the Sortilin:neurotensin complex at excess neurotensin concentration

Released:
DOI: 10.2210/pdb4po7/pdb
PDB entry 4po7 coloured by chain, front view.
PDB entry 4po7 coloured by chain, side view.
PDB entry 4po7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Revisiting the structure of the Vps10 domain of human sortilin and its interaction with neurotensin.
Quistgaard EM, Gr��ftehauge MK, Madsen P, Pallesen LT, Christensen B, S��rensen ES, Nissen P, Petersen CM, Thirup SS
Protein Sci. (2014)
PMID: 24985322

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151868 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Sortilin Chain: A
Molecule details ›
Chain: A
Length: 685 amino acids
Theoretical weight: 76.77 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q99523 (Residues: 78-756; Coverage: 85%)
Gene name: SORT1
Sequence domains:
Structure domains:
Neurotensin Chains: N, P
Molecule details ›
Chains: N, P
Length: 13 amino acids
Theoretical weight: 1.68 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P30990 (Residues: 151-163; Coverage: 9%)
Gene name: NTS

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand PG4 1 x PG4
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-5
Spacegroup: C2
Unit cell:
a: 162.01Å b: 78.65Å c: 110.98Å
α: 90° β: 126.62° γ: 90°
R-values:
R R work R free
0.168 0.166 0.212
Expression systems:
  • Cricetulus griseus
  • Not provided

Quick links

Citations

7 review citations

Sortilin, encoded by the cardiovascular risk gene SORT1, and its suggested functions in cardiovascular disease.
Kjolby et al. (2015)
6 more

4 mentions without citation

Phosphatidylinositol (3,4,5)-trisphosphate binds to sortilin and competes with neurotensin: Implications for very low density lipoprotein binding.
Sparks et al. (2016)
3 more