› 4prb

X-ray diffraction
1.75Å resolution

Crystal structure of a HLA-B*35:08-HPVG-A4

Released:
DOI: 10.2210/pdb4prb/pdb
PDB entry 4prb coloured by chain, front view.
PDB entry 4prb coloured by chain, side view.
PDB entry 4prb coloured by chain, top view.
Source organisms:
Primary publication:
A molecular basis for the interplay between T cells, viral mutants, and human leukocyte antigen micropolymorphism.
Liu YC, Chen Z, Neller MA, Miles JJ, Purcell AW, McCluskey J, Burrows SR, Rossjohn J, Gras S
J Biol Chem 289 16688-98 (2014)
PMID: 24759101

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-111520 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C5MK56 (Residues: 25-300; Coverage: 82%)
Gene name: HLA-B
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Epstein-Barr nuclear antigen 1 Chain: C
Molecule details ›
Chain: C
Length: 11 amino acids
Theoretical weight: 1.34 KDa
Source organism: Epstein-barr virus strain ag876
Expression system: Not provided
UniProt:
  • Canonical: Q1HVF7 (Residues: 407-417; Coverage: 2%)
Gene names: BKRF1, EBNA1

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P212121
Unit cell:
a: 50.8Å b: 82.1Å c: 110.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.201 0.234
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
5 more

2 mentions without citation

A molecular basis for the interplay between T cells, viral mutants, and human leukocyte antigen micropolymorphism.
Liu et al. (2014)
1 more