› 4puz

X-ray diffraction
2.08Å resolution

Crystal structure of spleen tyrosine kinase (Syk) in complex with GS-9973

Released:
DOI: 10.2210/pdb4puz/pdb
PDB entry 4puz coloured by chain, front view.
PDB entry 4puz coloured by chain, side view.
PDB entry 4puz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Discovery of GS-9973, a selective and orally efficacious inhibitor of spleen tyrosine kinase.
Currie KS, Kropf JE, Lee T, Blomgren P, Xu J, Zhao Z, Gallion S, Whitney JA, Maclin D, Lansdon EB, Maciejewski P, Rossi AM, Rong H, Macaluso J, Barbosa J, Di Paolo JA, Mitchell SA
J Med Chem 57 3856-73 (2014)
PMID: 24779514

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155056 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase SYK Chains: A, B
Molecule details ›
Chains: A, B
Length: 291 amino acids
Theoretical weight: 33.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P43405 (Residues: 353-635; Coverage: 45%)
Gene name: SYK
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CG9 2 x CG9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P1
Unit cell:
a: 39.882Å b: 41.877Å c: 87.405Å
α: 98.67° β: 90.43° γ: 101.38°
R-values:
R R work R free
0.213 0.21 0.261
Expression system: Spodoptera frugiperda

Quick links

Citations

21 review citations

Kinase inhibition in autoimmunity and inflammation.
Zarrin et al. (2021)
20 more

3 mentions without citation

Identification of Druggable Kinase Target Conformations Using Markov Model Metastable States Analysis of apo-Abl.
Paul et al. (2020)
2 more