› 4pvz

X-ray diffraction
2.5Å resolution

Structure of yeast importin a bound to the membrane protein Nuclear Localization Signal sequence of INM protein Heh2

Released:
DOI: 10.2210/pdb4pvz/pdb
PDB entry 4pvz coloured by chain, front view.
PDB entry 4pvz coloured by chain, side view.
PDB entry 4pvz coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2.
Lokareddy RK, Hapsari RA, van Rheenen M, Pumroy RA, Bhardwaj A, Steen A, Veenhoff LM, Cingolani G
Structure 23 1305-1316 (2015)
PMID: 26051712

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169772 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 422 amino acids
Theoretical weight: 46.79 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02821 (Residues: 88-509; Coverage: 78%)
Gene names: KAP60, N1606, SRP1, YNL189W
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Inner nuclear membrane protein HEH2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 43 amino acids
Theoretical weight: 4.98 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03281 (Residues: 100-137; Coverage: 6%)
Gene names: D8035.2, HEH2, YDR458C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P212121
Unit cell:
a: 49.47Å b: 105.32Å c: 224.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.226
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structural Biology and Regulation of Protein Import into the Nucleus.
Christie et al. (2016)
1 more

4 mentions without citation

Three-dimensional context rather than NLS amino acid sequence determines importin α subtype specificity for RCC1.
Sankhala et al. (2017)
3 more