› 4px9

X-ray diffraction
2.31Å resolution

DEAD-box RNA helicase DDX3X Domain 1 with N-terminal ATP-binding Loop

Released:
DOI: 10.2210/pdb4px9/pdb
PDB entry 4px9 coloured by chain, front view.
PDB entry 4px9 coloured by chain, side view.
PDB entry 4px9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cancer-associated mutants of RNA helicase DDX3X are defective in RNA-stimulated ATP hydrolysis.
Epling LB, Grace CR, Lowe BR, Partridge JF, Enemark EJ
J Mol Biol 427 1779-1796 (2015)
PMID: 25724843

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126356 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase DDX3X Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 292 amino acids
Theoretical weight: 33.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00571 (Residues: 135-407; Coverage: 41%)
Gene names: DBX, DDX3, DDX3X
Sequence domains: DEAD/DEAH box helicase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand ADP 3 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P31
Unit cell:
a: 75.637Å b: 75.637Å c: 121.388Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.255 0.255 0.285
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

DDX3, a potential target for cancer treatment.
Bol et al. (2015)
17 more

5 mentions without citation

Host DDX Helicases as Possible SARS-CoV-2 Proviral Factors: A Structural Overview of Their Hijacking Through Multiple Viral Proteins.
Squeglia et al. (2020)
4 more