› 4pxa

X-ray diffraction
3.2Å resolution

DEAD-box RNA helicase DDX3X Cancer-associated mutant D354V

Released:
DOI: 10.2210/pdb4pxa/pdb
PDB entry 4pxa coloured by chain, front view.
PDB entry 4pxa coloured by chain, side view.
PDB entry 4pxa coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cancer-associated mutants of RNA helicase DDX3X are defective in RNA-stimulated ATP hydrolysis.
Epling LB, Grace CR, Lowe BR, Partridge JF, Enemark EJ
J Mol Biol 427 1779-1796 (2015)
PMID: 25724843

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126356 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase DDX3X Chain: A
Molecule details ›
Chain: A
Length: 467 amino acids
Theoretical weight: 52.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00571 (Residues: 135-582; Coverage: 68%)
Gene names: DBX, DDX3, DDX3X
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand PO4 5 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43212
Unit cell:
a: 105.755Å b: 105.755Å c: 152.633Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.271
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

DDX3, a potential target for cancer treatment.
Bol et al. (2015)
16 more

9 mentions without citation

Host DDX Helicases as Possible SARS-CoV-2 Proviral Factors: A Structural Overview of Their Hijacking Through Multiple Viral Proteins.
Squeglia et al. (2020)
8 more