› 4pxf

X-ray diffraction
2.75Å resolution

Crystal structure of the active G-protein-coupled receptor opsin in complex with the finger-loop peptide derived from the full-length arrestin-1

Released:
DOI: 10.2210/pdb4pxf/pdb
PDB entry 4pxf coloured by chain, front view.
PDB entry 4pxf coloured by chain, side view.
PDB entry 4pxf coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal structure of a common GPCR-binding interface for G protein and arrestin.
Szczepek M, Beyrière F, Hofmann KP, Elgeti M, Kazmin R, Rose A, Bartl FJ, von Stetten D, Heck M, Sommer ME, Hildebrand PW, Scheerer P
Nat Commun 5 4801 (2014)
PMID: 25205354

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136080 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Rhodopsin Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 39.03 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P02699 (Residues: 1-348; Coverage: 100%)
Gene name: RHO
Sequence domains:
Structure domains: Rhodopsin 7-helix transmembrane proteins
S-arrestin short form Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.24 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P08168 (Residues: 67-77; Coverage: 3%)
Gene name: SAG

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
4 bound ligands:
Ligand BOG 3 x BOG
Ligand PLM 1 x PLM
Ligand SO4 1 x SO4
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: R32
Unit cell:
a: 242.622Å b: 242.622Å c: 110.216Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.215 0.251
Expression system: Not provided

Quick links

Citations

30 review citations

Biased signalling: from simple switches to allosteric microprocessors.
Smith et al. (2018)
29 more

16 mentions without citation

The Diverse Roles of Arrestin Scaffolds in G Protein-Coupled Receptor Signaling.
Peterson et al. (2017)
15 more