› 4q21

X-ray diffraction
2Å resolution

MOLECULAR SWITCH FOR SIGNAL TRANSDUCTION: STRUCTURAL DIFFERENCES BETWEEN ACTIVE AND INACTIVE FORMS OF PROTOONCOGENIC RAS PROTEINS

Released:
DOI: 10.2210/pdb4q21/pdb
PDB entry 4q21 coloured by chain, front view.
PDB entry 4q21 coloured by chain, side view.
PDB entry 4q21 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular switch for signal transduction: structural differences between active and inactive forms of protooncogenic ras proteins.
Milburn MV, Tong L, deVos AM, Brünger A, Yamaizumi Z, Nishimura S, Kim SH
Science 247 939-45 (1990)
PMID: 2406906

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 21.32 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01112 (Residues: 1-189; Coverage: 100%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I4
Unit cell:
a: 96.3Å b: 96.3Å c: 41.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 not available
Expression system: Not provided

Quick links

Citations

130 review citations

The GTPase superfamily: conserved structure and molecular mechanism.
Bourne et al. (1991)
129 more

215 mentions without citation

Ras oncogenes: split personalities.
Karnoub et al. (2008)
214 more