› 4q6f

X-ray diffraction
1.91Å resolution

Crystal structure of human BAZ2A PHD zinc finger in complex with unmodified H3K4 histone peptide

Released:
DOI: 10.2210/pdb4q6f/pdb
PDB entry 4q6f coloured by chain, front view.
PDB entry 4q6f coloured by chain, side view.
PDB entry 4q6f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.
Tallant C, Valentini E, Fedorov O, Overvoorde L, Ferguson FM, Filippakopoulos P, Svergun DI, Knapp S, Ciulli A
Structure 23 80-92 (2015)
PMID: 25533489

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-165074 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bromodomain adjacent to zinc finger domain protein 2A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 58 amino acids
Theoretical weight: 6.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UIF9 (Residues: 1673-1728; Coverage: 3%)
Gene names: BAZ2A, KIAA0314, TIP5
Sequence domains: PHD-finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
unmodified H3K4 peptide Chains: F, G, H
Molecule details ›
Chains: F, G, H
Length: 5 amino acids
Theoretical weight: 605 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 8 x ZN
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P43212
Unit cell:
a: 71.866Å b: 71.866Å c: 98.767Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.219
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

16 review citations

Mechanisms of action and regulation of ATP-dependent chromatin-remodelling complexes.
Clapier et al. (2017)
15 more

5 mentions without citation

Atypical histone targets of PHD fingers.
Black et al. (2023)
4 more