› 4q94

X-ray diffraction
1.85Å resolution

human RPRD1B CID in complex with a RPB1-CTD derived Ser2 phosphorylated peptide

Released:
DOI: 10.2210/pdb4q94/pdb
PDB entry 4q94 coloured by chain, front view.
PDB entry 4q94 coloured by chain, side view.
PDB entry 4q94 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation.
Ni Z, Xu C, Guo X, Hunter GO, Kuznetsova OV, Tempel W, Marcon E, Zhong G, Guo H, Kuo WW, Li J, Young P, Olsen JB, Wan C, Loppnau P, El Bakkouri M, Senisterra GA, He H, Huang H, Sidhu SS, Emili A, Murphy S, Mosley AL, Arrowsmith CH, Min J, Greenblatt JF
Nat Struct Mol Biol 21 686-695 (2014)
PMID: 24997600

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-165081 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Regulation of nuclear pre-mRNA domain-containing protein 1B Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 15.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NQG5 (Residues: 2-135; Coverage: 41%)
Gene names: C20orf77, CREPT, RPRD1B
Sequence domains: CID domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
rpb1-ctd Chains: C, D
Molecule details ›
Chains: C, D
Length: 21 amino acids
Theoretical weight: 2.36 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand UNX 19 x UNX
1 modified residue:
Ligand SEP 4 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 39.286Å b: 70.448Å c: 108.859Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.221
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

14 review citations

The pol II CTD: new twists in the tail.
Zaborowska et al. (2016)
13 more

3 mentions without citation

What's all the phos about? Insights into the phosphorylation state of the RNA polymerase II C-terminal domain via mass spectrometry.
LeBlanc et al. (2021)
2 more