› 4qbe

X-ray diffraction
2.29Å resolution

The second sphere residue T263 is important for function and activity of PTP1B through modulating WPD loop

Released:
DOI: 10.2210/pdb4qbe/pdb
PDB entry 4qbe coloured by chain, front view.
PDB entry 4qbe coloured by chain, side view.
PDB entry 4qbe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The second-sphere residue T263 is important for the function and catalytic activity of PTP1B via interaction with the WPD-loop.
Xiao P, Wang X, Wang HM, Fu XL, Cui FA, Yu X, Wen SS, Bi WX, Sun JP
Int J Biochem Cell Biol 57 84-95 (2014)
PMID: 25450460

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 34.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18031 (Residues: 1-298; Coverage: 69%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3121
Unit cell:
a: 88.452Å b: 88.452Å c: 103.788Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.189 0.23
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Frequent activating STAT3 mutations and novel recurrent genomic abnormalities detected in breast implant-associated anaplastic large cell lymphoma.
Blombery et al. (2018)
6 more

1 mention without citation

Native dynamics and allosteric responses in PTP1B probed by high-resolution HDX-MS.
Woods et al. (2023)