› 4qbm

X-ray diffraction
1.65Å resolution

Crystal structure of human BAZ2A bromodomain in complex with a diacetylated histone 4 peptide (H4K16acK20ac)

Released:
DOI: 10.2210/pdb4qbm/pdb
PDB entry 4qbm coloured by chain, front view.
PDB entry 4qbm coloured by chain, side view.
PDB entry 4qbm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.
Tallant C, Valentini E, Fedorov O, Overvoorde L, Ferguson FM, Filippakopoulos P, Svergun DI, Knapp S, Ciulli A
Structure 23 80-92 (2015)
PMID: 25533489

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165089 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bromodomain adjacent to zinc finger domain protein 2A Chains: A, B
Molecule details ›
Chains: A, B
Length: 106 amino acids
Theoretical weight: 12.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UIF9 (Residues: 1796-1899; Coverage: 6%)
Gene names: BAZ2A, KIAA0314, TIP5
Sequence domains: Bromodomain
Structure domains: Bromodomain-like
histone H4 peptide with sequence Gly-Ala-Lys(ac)-Arg-His-Arg-Lys(ac)-Val-Leu Chains: C, D
Molecule details ›
Chains: C, D
Length: 9 amino acids
Theoretical weight: 1.15 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
1 modified residue:
Ligand ALY 4 x ALY

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P41
Unit cell:
a: 64.869Å b: 64.869Å c: 71.637Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.205
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

16 review citations

Mechanisms of action and regulation of ATP-dependent chromatin-remodelling complexes.
Clapier et al. (2017)
15 more

6 mentions without citation

Predictions of Ligand Selectivity from Absolute Binding Free Energy Calculations.
Aldeghi et al. (2017)
5 more