› 4qg4

X-ray diffraction
2.1Å resolution

Crystal structure of the tetrameric GTP/dATP/ATP-bound SAMHD1 (H210A) mutant catalytic core

Released:
DOI: 10.2210/pdb4qg4/pdb
PDB entry 4qg4 coloured by chain, front view.
PDB entry 4qg4 coloured by chain, side view.
PDB entry 4qg4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of allosteric activation of sterile α motif and histidine-aspartate domain-containing protein 1 (SAMHD1) by nucleoside triphosphates.
Koharudin LM, Wu Y, DeLucia M, Mehrens J, Gronenborn AM, Ahn J
J Biol Chem 289 32617-27 (2014)
PMID: 25288794

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195223 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 550 amino acids
Theoretical weight: 63.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y3Z3 (Residues: 113-626; Coverage: 82%)
Gene names: MOP5, SAMHD1
Sequence domains: HD domain
Structure domains: Hypothetical protein af1432

Ligands and Environments

4 bound ligands:
Ligand GTP 4 x GTP
Ligand DTP 4 x DTP
Ligand MG 4 x MG
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 87.799Å b: 147.12Å c: 99.211Å
α: 90° β: 114° γ: 90°
R-values:
R R work R free
0.228 0.222 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Deoxyribonucleotide metabolism, mutagenesis and cancer.
Mathews CK. (2015)
10 more