› 4qjr

X-ray diffraction
2.4Å resolution

Crystal structure of human nuclear receptor sf-1 (nr5a1) bound to its hormone pip3 at 2.4 a resolution

Released:
DOI: 10.2210/pdb4qjr/pdb
PDB entry 4qjr coloured by chain, front view.
PDB entry 4qjr coloured by chain, side view.
PDB entry 4qjr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The signaling phospholipid PIP3 creates a new interaction surface on the nuclear receptor SF-1.
Blind RD, Sablin EP, Kuchenbecker KM, Chiu HJ, Deacon AM, Das D, Fletterick RJ, Ingraham HA
Proc Natl Acad Sci U S A 111 15054-9 (2014)
PMID: 25288771

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171630 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Steroidogenic factor 1 Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 27.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13285 (Residues: 218-461; Coverage: 53%)
Gene names: AD4BP, FTZF1, NR5A1, SF1
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha Chain: B
Molecule details ›
Chain: B
Length: 14 amino acids
Theoretical weight: 1.52 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9UBK2 (Residues: 139-152; Coverage: 2%)
Gene names: LEM6, PGC1, PGC1A, PPARGC1, PPARGC1A

Ligands and Environments

2 bound ligands:
Ligand PIZ 1 x PIZ
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P41212
Unit cell:
a: 74.85Å b: 74.85Å c: 139.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.23
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

15 review citations

DAX-1 (NR0B1) and steroidogenic factor-1 (SF-1, NR5A1) in human disease.
Suntharalingham et al. (2015)
14 more

16 mentions without citation

How is the acyl chain composition of phosphoinositides created and does it matter?
Barneda et al. (2019)
15 more