› 4qk3

X-ray diffraction
1.34Å resolution

Structural and Catalytic Effects of Proline Substitution and Surface Loop Deletion in the Extended Active Site of Human Carbonic Anhydrase II - [delta]230-240

Released:
DOI: 10.2210/pdb4qk3/pdb
PDB entry 4qk3 coloured by chain, front view.
PDB entry 4qk3 coloured by chain, side view.
PDB entry 4qk3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and catalytic effects of proline substitution and surface loop deletion in the extended active site of human carbonic anhydrase II.
Boone CD, Rasi V, Tu C, McKenna R
FEBS J 282 1445-57 (2015)
PMID: 25683338

Function and Biology Details

Reactions catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Urea = cyanamide + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133681 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 2 Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 28.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00918 (Residues: 1-260; Coverage: 96%)
Gene name: CA2
Sequence domains: Eukaryotic-type carbonic anhydrase
Structure domains: Alpha carbonic anhydrase

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 42.05Å b: 69.018Å c: 74.168Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.217 0.25
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Physical and molecular bases of protein thermal stability and cold adaptation.
Pucci et al. (2017)
2 more

1 mention without citation

Structural and catalytic effects of proline substitution and surface loop deletion in the extended active site of human carbonic anhydrase II.
Boone et al. (2015)