› 4qkx

X-ray diffraction
3.3Å resolution

Structure of beta2 adrenoceptor bound to a covalent agonist and an engineered nanobody

Released:
DOI: 10.2210/pdb4qkx/pdb
PDB entry 4qkx coloured by chain, front view.
PDB entry 4qkx coloured by chain, side view.
PDB entry 4qkx coloured by chain, top view.
Source organisms:
Primary publication:
Covalent agonists for studying G protein-coupled receptor activation.
Weichert D, Kruse AC, Manglik A, Hiller C, Zhang C, Hübner H, Kobilka BK, Gmeiner P
Proc Natl Acad Sci U S A 111 10744-8 (2014)
PMID: 25006259

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208293 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta-2 adrenergic receptor; Endolysin Chain: A
Molecule details ›
Chain: A
Length: 469 amino acids
Theoretical weight: 53.44 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00720 (Residues: 2-161; Coverage: 98%)
  • Canonical: P07550 (Residues: 29-348; Coverage: 71%)
Gene names: ADRB2, ADRB2R, B2AR, E
Sequence domains:
R9 protein Chain: B
Molecule details ›
Chain: B
Length: 120 amino acids
Theoretical weight: 12.95 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand NA 2 x NA
Ligand 35V 1 x 35V
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 49.558Å b: 67.211Å c: 301.269Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.236 0.28
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Methodological advances: the unsung heroes of the GPCR structural revolution.
Ghosh et al. (2015)
11 more

14 mentions without citation

Structural Basis for G Protein-Coupled Receptor Activation.
Manglik et al. (2017)
13 more