› 4qlk

X-ray diffraction
1.83Å resolution

Crystal structure of rice BGlu1 E176Q/Y341A mutant complexed with cellotetraose

Released:
DOI: 10.2210/pdb4qlk/pdb
PDB entry 4qlk coloured by chain, front view.
PDB entry 4qlk coloured by chain, side view.
PDB entry 4qlk coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Effects of active site cleft residues on oligosaccharide binding, hydrolysis, and glycosynthase activities of rice BGlu1 and its mutants.
Pengthaisong S, Ketudat Cairns JR
Protein Sci 23 1738-52 (2014)
PMID: 25252199

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181114 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-glucosidase 7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 481 amino acids
Theoretical weight: 54.64 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q75I93 (Residues: 29-504; Coverage: 100%)
Gene names: BGLU1, BGLU7, LOC_Os03g49600, OSJNBa0004L11.16, Os03g0703000
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
3 bound ligands:
Ligand ZN 1 x ZN
Ligand SO4 2 x SO4
Ligand MES 2 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21
Unit cell:
a: 63.66Å b: 79.531Å c: 101.989Å
α: 90° β: 97.83° γ: 90°
R-values:
R R work R free
0.153 0.152 0.169
Expression system: Escherichia coli

Quick links

Citations

1 review citation

β-Glucosidases: Multitasking, moonlighting or simply misunderstood?
Ketudat Cairns et al. (2015)
4 other articles

4 mentions without citation

Integrating Systems and Synthetic Biology to Understand and Engineer Microbiomes.
Leggieri et al. (2021)
3 more