› 4qn9

X-ray diffraction
2.65Å resolution

Function and Biology Details

Reaction catalysed: 
An N-acylphosphatidylethanolamine + H(2)O = an N-acylethanolamine + a 1,2-diacylglycerol 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179769 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D Chains: A, B
Molecule details ›
Chains: A, B
Length: 393 amino acids
Theoretical weight: 45.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6IQ20 (Residues: 1-393; Coverage: 100%)
Gene names: C7orf18, NAPEPLD
Sequence domains: Beta-lactamase superfamily domain

Ligands and Environments

4 bound ligands:
Ligand ZN 4 x ZN
Ligand 3PE 2 x 3PE
Ligand DXC 11 x DXC
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P6522
Unit cell:
a: 95.099Å b: 95.099Å c: 444.17Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.214 0.253
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Secondary Bile Acids and Short Chain Fatty Acids in the Colon: A Focus on Colonic Microbiome, Cell Proliferation, Inflammation, and Cancer.
Zeng et al. (2019)
17 more

10 mentions without citation

Characterization of Lipid-Protein Interactions and Lipid-Mediated Modulation of Membrane Protein Function through Molecular Simulation.
Muller et al. (2019)
9 more