› 4qnh

X-ray diffraction
2.02Å resolution

Calcium-calmodulin (T79D) complexed with the calmodulin binding domain from a small conductance potassium channel SK2-a

Released:
DOI: 10.2210/pdb4qnh/pdb
PDB entry 4qnh coloured by chain, front view.
PDB entry 4qnh coloured by chain, side view.
PDB entry 4qnh coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Selective phosphorylation modulates the PIP2 sensitivity of the CaM-SK channel complex.
Zhang M, Meng XY, Cui M, Pascal JM, Logothetis DE, Zhang JF
Nat Chem Biol 10 753-9 (2014)
PMID: 25108821

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159744 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Small conductance calcium-activated potassium channel protein 2 Chain: B
Molecule details ›
Chain: B
Length: 102 amino acids
Theoretical weight: 12.24 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P70604 (Residues: 394-394, 396-487; Coverage: 16%)
Gene name: Kcnn2
Sequence domains: Calmodulin binding domain
Structure domains: Helix Hairpins
Calmodulin Chain: R
Molecule details ›
Chain: R
Length: 149 amino acids
Theoretical weight: 16.87 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2
Unit cell:
a: 77.16Å b: 65.96Å c: 64.68Å
α: 90° β: 93.56° γ: 90°
R-values:
R R work R free
0.184 0.182 0.234
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Modulation of Kv7 channels and excitability in the brain.
Greene et al. (2017)
19 more

3 mentions without citation

Structural insight into substrate and inhibitor discrimination by human P-glycoprotein.
Alam et al. (2019)
2 more