› 4qxr

X-ray diffraction
2.37Å resolution

Crystal structure of hSTING(S162A/G230I/Q266I) in complex with DMXAA

Released:
DOI: 10.2210/pdb4qxr/pdb
PDB entry 4qxr coloured by chain, front view.
PDB entry 4qxr coloured by chain, side view.
PDB entry 4qxr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Binding-pocket and lid-region substitutions render human STING sensitive to the species-specific drug DMXAA.
Gao P, Zillinger T, Wang W, Ascano M, Dai P, Hartmann G, Tuschl T, Deng L, Barchet W, Patel DJ
Cell Rep 8 1668-1676 (2014)
PMID: 25199835

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183240 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Stimulator of interferon genes protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 21.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q86WV6 (Residues: 155-341; Coverage: 49%)
Gene names: ERIS, MITA, STING, STING1, TMEM173
Sequence domains: Transmembrane protein 173
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 1YE 2 x 1YE
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P6
Unit cell:
a: 148.576Å b: 148.576Å c: 36.202Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.187 0.231
Expression system: Escherichia coli

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Citations

18 review citations

Cyclic GMP-AMP as an Endogenous Second Messenger in Innate Immune Signaling by Cytosolic DNA.
Kato et al. (2017)
17 more

4 mentions without citation

Host-pathogen protein-nucleic acid interactions: A comprehensive review.
Jain et al. (2022)
3 more