Function and Biology Details
Reactions catalysed:
ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)
ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Arginine-tRNA ligase
- Glutamyl/glutaminyl-tRNA synthetase
- Large ribosomal subunit protein bL25/Gln-tRNA synthetase, N-terminal
- Aminoacyl-tRNA synthetase, class I, conserved site
- Rossmann-like alpha/beta/alpha sandwich fold
- Arginyl-tRNA synthetase, catalytic core domain
- Arginyl tRNA synthetase N-terminal domain
- Arginyl tRNA synthetase N-terminal domain superfamily
12 more domains
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-155673 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Aminoacyl tRNA synthase complex-interacting multifunctional protein 1
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 34.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Putative tRNA binding domain
Length: 312 amino acids
Theoretical weight: 34.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
Q12904 (Residues: 1-312; Coverage: 100%)
Sequence domains: Putative tRNA binding domain
Arginine--tRNA ligase, cytoplasmic
Molecule details ›
Chain: B
Length: 675 amino acids
Theoretical weight: 77.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 675 amino acids
Theoretical weight: 77.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: P54136 (Residues: 1-660; Coverage: 100%)
Sequence domains:
Glutamine--tRNA ligase
Molecule details ›
Chain: C
Length: 799 amino acids
Theoretical weight: 90.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 799 amino acids
Theoretical weight: 90.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: P47897 (Residues: 1-775; Coverage: 100%)
Sequence domains:
- Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
- Glutaminyl-tRNA synthetase, non-specific RNA binding region part 2
- tRNA synthetases class I (E and Q), catalytic domain
- tRNA synthetases class I (E and Q), anti-codon binding domain
- tRNA synthetases class I (E and Q), anti-codon binding domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
PAL/PLS BEAMLINE 5C (4A)
Spacegroup:
C2221
Expression system: Escherichia coli
