› 4r4c

X-ray diffraction
1.4Å resolution

Structure of RPA70N in complex with 5-(4-((4-(5-carboxyfuran-2-yl)-2-chlorobenzamido)methyl)phenyl)-1-(3,4-dichlorophenyl)-1H-pyrazole-3-carboxylic acid

Released:
DOI: 10.2210/pdb4r4c/pdb
PDB entry 4r4c coloured by chain, front view.
PDB entry 4r4c coloured by chain, side view.
PDB entry 4r4c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Diphenylpyrazoles as replication protein a inhibitors.
Waterson AG, Kennedy JP, Patrone JD, Pelz NF, Feldkamp MD, Frank AO, Vangamudi B, Souza-Fagundes EM, Rossanese OW, Chazin WJ, Fesik SW
ACS Med Chem Lett 6 140-5 (2015)
PMID: 25699140

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151049 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replication protein A 70 kDa DNA-binding subunit, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 13.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27694 (Residues: 1-120; Coverage: 20%)
Gene names: REPA1, RPA1, RPA70
Sequence domains: Replication factor-A protein 1, N-terminal domain
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

1 bound ligand:
Ligand 3HS 1 x 3HS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 38.07Å b: 53.864Å c: 53.977Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.148 0.189
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

DNA repair targeted therapy: The past or future of cancer treatment?
Gavande et al. (2016)
3 more

1 mention without citation

Diphenylpyrazoles as replication protein a inhibitors.
Waterson et al. (2015)