› 4r9t

X-ray diffraction
2.25Å resolution

L-ficolin complexed to sulphates

Released:
DOI: 10.2210/pdb4r9t/pdb
PDB entry 4r9t coloured by chain, front view.
PDB entry 4r9t coloured by chain, side view.
PDB entry 4r9t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human ficolin-2 recognition versatility extended: an update on the binding of ficolin-2 to sulfated/phosphated carbohydrates.
Laffly E, Lacroix M, Martin L, Vassal-Stermann E, Thielens NM, Gaboriaud C
FEBS Lett 588 4694-700 (2014)
PMID: 25447524

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172307 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Ficolin-2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 218 amino acids
Theoretical weight: 24.73 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: Q15485 (Residues: 97-313; Coverage: 75%)
Gene names: FCN2, FCNL
Sequence domains: Fibrinogen beta and gamma chains, C-terminal globular domain
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand ACT 2 x ACT
Ligand CA 3 x CA
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3221
Unit cell:
a: 96.11Å b: 96.11Å c: 141.19Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.188 0.218
Expression system: unidentified baculovirus

Quick links

Citations

2 review citations

Recognition of microbial glycans by soluble human lectins.
Wesener et al. (2017)
1 more