› 4r9y

X-ray diffraction
4.11Å resolution

Crystal structure of KKOFab in complex with platelet factor 4

Released:
DOI: 10.2210/pdb4r9y/pdb
PDB entry 4r9y coloured by chain, front view.
PDB entry 4r9y coloured by chain, side view.
PDB entry 4r9y coloured by chain, top view.
Source organisms:
Primary publication:
Atomic description of the immune complex involved in heparin-induced thrombocytopenia.
Cai Z, Yarovoi SV, Zhu Z, Rauova L, Hayes V, Lebedeva T, Liu Q, Poncz M, Arepally G, Cines DB, Greene MI
Nat Commun 6 8277 (2015)
PMID: 26391892

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208790 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Platelet factor 4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 70 amino acids
Theoretical weight: 7.78 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P02776 (Residues: 32-101; Coverage: 100%)
Gene names: CXCL4, PF4, SCYB4
Sequence domains: Small cytokines (intecrine/chemokine), interleukin-8 like
Platelet factor 4 antibody KKO light chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 214 amino acids
Theoretical weight: 23.73 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Platelet factor 4 antibody KKO heavy chain Chains: H, N
Molecule details ›
Chains: H, N
Length: 218 amino acids
Theoretical weight: 23.32 KDa
Source organism: Mus musculus
Expression system: Mus musculus

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P212121
Unit cell:
a: 49.49Å b: 99.34Å c: 261.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.32 0.317 0.382
Expression systems:
  • Drosophila melanogaster
  • Mus musculus

Quick links

Citations

22 review citations

Heparin-induced thrombocytopenia.
Arepally GM. (2017)
21 more

4 mentions without citation

Atomic features of an autoantigen in heparin-induced thrombocytopenia (HIT).
Cai et al. (2016)
3 more