› 4reb

X-ray diffraction
4.2Å resolution

Structural Insights into 5' Flap DNA Unwinding and Incision by the Human FAN1 Dimer

Released:
DOI: 10.2210/pdb4reb/pdb
PDB entry 4reb coloured by chain, front view.
PDB entry 4reb coloured by chain, side view.
PDB entry 4reb coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into 5' flap DNA unwinding and incision by the human FAN1 dimer.
Zhao Q, Xue X, Longerich S, Sung P, Xiong Y
Nat Commun 5 5726 (2014)
PMID: 25500724

Function and Biology Details

Reaction catalysed: 
Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118095 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Fanconi-associated nuclease 1 Chains: A, H
Molecule details ›
Chains: A, H
Length: 647 amino acids
Theoretical weight: 73.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y2M0 (Residues: 373-1017; Coverage: 63%)
Gene names: FAN1, KIAA1018, MTMR15
Sequence domains:
DNA (5'-D(P*GP*TP*GP*GP*CP*GP*AP*GP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 9 nucleotides
Theoretical weight: 2.8 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*CP*GP*TP*GP*GP*CP*GP*AP*GP*CP*GP*CP*TP*CP*GP*CP*CP*AP*CP*G)-3') Chain: E
Molecule details ›
Chain: E
Length: 20 nucleotides
Theoretical weight: 6.14 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*GP*CP*TP*CP*GP*CP*CP*AP*CP*G)-3') Chain: F
Molecule details ›
Chain: F
Length: 10 nucleotides
Theoretical weight: 3.01 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SM 2 x SM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P31
Unit cell:
a: 104.77Å b: 104.77Å c: 127.55Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.23 0.262
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

The Fanconi anaemia pathway: new players and new functions.
Ceccaldi et al. (2016)
5 more

1 mention without citation

Structural insights into 5' flap DNA unwinding and incision by the human FAN1 dimer.
Zhao et al. (2014)