4rey

X-ray diffraction
1.96Å resolution

Crystal Structure of the GRASP65-GM130 C-terminal peptide complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-170639 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Golgi reassembly-stacking protein 1 Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 23.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BQQ3 (Residues: 2-210; Coverage: 48%)
Gene names: GOLPH5, GORASP1, GRASP65
Sequence domains: GRASP55/65 PDZ-like domain
Structure domains: Pdz3 Domain
Golgin subfamily A member 2 Chain: B
Molecule details ›
Chain: B
Length: 27 amino acids
Theoretical weight: 3.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q08379 (Residues: 980-1002; Coverage: 2%)
Gene name: GOLGA2
Sequence domains: GM130 C-terminal binding motif

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P61
Unit cell:
a: 129.725Å b: 129.725Å c: 37.104Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.155 0.152 0.19
Expression system: Escherichia coli BL21(DE3)