› 4rh3

X-ray diffraction
3.02Å resolution

AMPPCP-bound structure of human platelet phosphofructokinase in an R-state, crystal form II

Released:
DOI: 10.2210/pdb4rh3/pdb
PDB entry 4rh3 coloured by chain, front view.
PDB entry 4rh3 coloured by chain, side view.
PDB entry 4rh3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human platelet phosphofructokinase-1 locked in an activated conformation.
Kloos M, Brüser A, Kirchberger J, Schöneberg T, Sträter N
Biochem J 469 421-32 (2015)
PMID: 26205495

Function and Biology Details

Reaction catalysed: 
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169632 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent 6-phosphofructokinase, platelet type Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 743 amino acids
Theoretical weight: 81.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01813 (Residues: 26-762; Coverage: 94%)
Gene names: PFKF, PFKP
Sequence domains: Phosphofructokinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACP 4 x ACP
Ligand PO4 7 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P3221
Unit cell:
a: 132.911Å b: 132.911Å c: 397.168Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.148 0.146 0.187
Expression system: Escherichia coli

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Citations

3 review citations

Protein Ensembles: How Does Nature Harness Thermodynamic Fluctuations for Life? The Diverse Functional Roles of Conformational Ensembles in the Cell.
Wei et al. (2016)
2 more

1 mention without citation

Identification of Alternative Allosteric Sites in Glycolytic Enzymes for Potential Use as Species-Specific Drug Targets.
Ayyildiz et al. (2020)