› 4ri8

X-ray diffraction
2.9Å resolution

FAN1 Nuclease bound to 5' phosphorylated p(dG)/3'(dT-dT-dT-dT) double flap DNA

Released:
DOI: 10.2210/pdb4ri8/pdb
PDB entry 4ri8 coloured by chain, front view.
PDB entry 4ri8 coloured by chain, side view.
PDB entry 4ri8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
DNA repair. Mechanism of DNA interstrand cross-link processing by repair nuclease FAN1.
Wang R, Persky NS, Yoo B, Ouerfelli O, Smogorzewska A, Elledge SJ, Pavletich NP
Science 346 1127-30 (2014)
PMID: 25430771

Function and Biology Details

Reaction catalysed: 
Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118098 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Fanconi-associated nuclease 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 651 amino acids
Theoretical weight: 73.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y2M0 (Residues: 370-1017; Coverage: 63%)
Gene names: FAN1, KIAA1018, MTMR15
Sequence domains:
DNA (5'-D(*TP*TP*TP*GP*AP*GP*GP*AP*GP*TP*CP*TP*TP*T)-3') Chains: E, H
Molecule details ›
Chains: E, H
Length: 14 nucleotides
Theoretical weight: 4.32 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*GP*AP*GP*GP*CP*GP*TP*G)-3') Chains: F, I
Molecule details ›
Chains: F, I
Length: 8 nucleotides
Theoretical weight: 2.51 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*AP*AP*CP*AP*CP*GP*CP*CP*TP*AP*GP*AP*CP*TP*CP*CP*TP*CP*A)-3') Chains: G, J
Molecule details ›
Chains: G, J
Length: 19 nucleotides
Theoretical weight: 5.72 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 62.26Å b: 210.88Å c: 83.22Å
α: 90° β: 107.61° γ: 90°
R-values:
R R work R free
0.222 0.221 0.255
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

13 review citations

Fanconi anaemia and cancer: an intricate relationship.
Nalepa et al. (2018)
12 more

4 mentions without citation

Structure and mechanism of the Red recombination system of bacteriophage λ.
Caldwell et al. (2019)
3 more