› 4rjt

X-ray diffraction
2.7Å resolution

Crystal Structure of Unliganded, Full Length hUGDH at pH 7.0

Released:
DOI: 10.2210/pdb4rjt/pdb
PDB entry 4rjt coloured by chain, front view.
PDB entry 4rjt coloured by chain, side view.
PDB entry 4rjt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Hysteresis in human UDP-glucose dehydrogenase is due to a restrained hexameric structure that favors feedback inhibition.
Kadirvelraj R, Custer GS, Keul ND, Sennett NC, Sidlo AM, Walsh RM, Wood ZA
Biochemistry 53 8043-51 (2014)
PMID: 25478983

Function and Biology Details

Reaction catalysed: 
UDP-glucose + 2 NAD(+) + H(2)O = UDP-glucuronate + 2 NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130073 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucose 6-dehydrogenase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 494 amino acids
Theoretical weight: 55.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60701 (Residues: 1-494; Coverage: 100%)
Gene name: UGDH
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: C2
Unit cell:
a: 176.98Å b: 113.96Å c: 97.12Å
α: 90° β: 116.91° γ: 90°
R-values:
R R work R free
0.185 0.183 0.23
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Integration of Sugar Metabolism and Proteoglycan Synthesis by UDP-glucose Dehydrogenase.
Zimmer et al. (2021)
2 more

5 mentions without citation

The entropic force generated by intrinsically disordered segments tunes protein function.
Keul et al. (2018)
4 more