› 4rmq

X-ray diffraction
1.46Å resolution

Crystal structure of the D59N Beta-2 Microglobulin mutant

Released:
DOI: 10.2210/pdb4rmq/pdb
PDB entry 4rmq coloured by chain, front view.
PDB entry 4rmq coloured by chain, side view.
PDB entry 4rmq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Decoding the Structural Bases of D76N ß2-Microglobulin High Amyloidogenicity through Crystallography and Asn-Scan Mutagenesis.
de Rosa M, Barbiroli A, Giorgetti S, Mangione PP, Bolognesi M, Ricagno S
PLoS One 10 e0144061 (2015)
PMID: 26625273

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-2-microglobulin Chain: A
Molecule details ›
Chain: A
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Unit cell:
a: 54.77Å b: 28.93Å c: 67.256Å
α: 90° β: 101.76° γ: 90°
R-values:
R R work R free
0.169 0.166 0.193
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Early Phase of β2-Microglobulin Aggregation: Perspectives From Molecular Simulations.
Loureiro et al. (2020)
14 other articles

1 mention without citation

Decoding the Structural Bases of D76N ß2-Microglobulin High Amyloidogenicity through Crystallography and Asn-Scan Mutagenesis.
de Rosa et al. (2015)