› 4rqt

X-ray diffraction
2.3Å resolution

Alcohol Dehydrogenase Crystal Structure

Released:
DOI: 10.2210/pdb4rqt/pdb
PDB entry 4rqt coloured by chain, front view.
PDB entry 4rqt coloured by chain, side view.
PDB entry 4rqt coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural insight into the conformational change of alcohol dehydrogenase from Arabidopsis thaliana L. during coenzyme binding.
Chen F, Wang P, An Y, Huang J, Xu Y
Biochimie 108 33-9 (2015)
PMID: 25447145

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139070 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase class-P Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 40.84 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06525 (Residues: 6-379; Coverage: 99%)
Gene names: ADH, ADH1, At1g77120, F22K20.19
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand SO4 3 x SO4
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P6122
Unit cell:
a: 103.604Å b: 103.604Å c: 168.127Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.165 0.162 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 citation in other articles

Alcohol dehydrogenase 1 (ADH1) confers both abiotic and biotic stress resistance in Arabidopsis.
Shi et al. (2017)
5 more

1 mention without citation

Conservation of ethanol fermentation and its regulation in land plants.
Bui et al. (2019)