› 4rqu

X-ray diffraction
2.5Å resolution

Alcohol Dehydrogenase crystal structure in complex with NAD

Released:
DOI: 10.2210/pdb4rqu/pdb
PDB entry 4rqu coloured by chain, front view.
PDB entry 4rqu coloured by chain, side view.
PDB entry 4rqu coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural insight into the conformational change of alcohol dehydrogenase from Arabidopsis thaliana L. during coenzyme binding.
Chen F, Wang P, An Y, Huang J, Xu Y
Biochimie 108 33-9 (2015)
PMID: 25447145

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139070 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase class-P Chains: A, B
Molecule details ›
Chains: A, B
Length: 375 amino acids
Theoretical weight: 40.84 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06525 (Residues: 6-379; Coverage: 99%)
Gene names: ADH, ADH1, At1g77120, F22K20.19
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3121
Unit cell:
a: 101.15Å b: 101.15Å c: 165.64Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.17 0.198
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 citation in other articles

Mechanistic implications from structures of yeast alcohol dehydrogenase complexed with coenzyme and an alcohol.
Plapp et al. (2016)
6 more