› 4rqx

X-ray diffraction
2.26Å resolution

Crystal structure of human peroxiredoxin 4(THIOREDOXIN PEROXIDASE) with MESNA

Released:
DOI: 10.2210/pdb4rqx/pdb
PDB entry 4rqx coloured by chain, front view.
PDB entry 4rqx coloured by chain, side view.
PDB entry 4rqx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cysteine specific targeting of the functionally distinct peroxiredoxin and glutaredoxin proteins by the investigational disulfide BNP7787.
Parker AR, Petluru PN, Nienaber VL, Badger J, Leverett BD, Jair K, Sridhar V, Logan C, Ayala PY, Kochat H, Hausheer FH
Molecules 20 4928-50 (2015)
PMID: 25793542

Function and Biology Details

Reaction catalysed: 
Thioredoxin + ROOH = thioredoxin disulfide + H(2)O + ROH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171577 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxiredoxin-4 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 226 amino acids
Theoretical weight: 25.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13162 (Residues: 79-271; Coverage: 83%)
Gene name: PRDX4
Sequence domains:
Structure domains: Glutaredoxin

Ligands and Environments

1 bound ligand:
Ligand COM 5 x COM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2
Unit cell:
a: 108.004Å b: 139.684Å c: 96.191Å
α: 90° β: 103.17° γ: 90°
R-values:
R R work R free
0.21 0.208 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Thiol-based redox-active proteins as cardioprotective therapeutic agents in cardiovascular diseases.
Andreadou et al. (2021)
5 other articles

2 mentions without citation

Peroxiredoxins wear many hats: Factors that fashion their peroxide sensing personalities.
Bolduc et al. (2021)
1 more