› 4rxo

X-ray diffraction
2.6Å resolution

The structure of GTP-bound SAMHD1

Released:
DOI: 10.2210/pdb4rxo/pdb
PDB entry 4rxo coloured by chain, front view.
PDB entry 4rxo coloured by chain, side view.
PDB entry 4rxo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The mechanism of substrate-controlled allosteric regulation of SAMHD1 activated by GTP.
Zhu CF, Wei W, Peng X, Dong YH, Gong Y, Yu XF
Acta Crystallogr D Biol Crystallogr 71 516-24 (2015)
PMID: 25760601

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195221 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 539 amino acids
Theoretical weight: 62.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y3Z3 (Residues: 109-626; Coverage: 83%)
Gene names: MOP5, SAMHD1
Sequence domains: HD domain
Structure domains: Hypothetical protein af1432

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand PO4 4 x PO4
Ligand GTP 4 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P21
Unit cell:
a: 77.219Å b: 183.187Å c: 81.275Å
α: 90° β: 100.62° γ: 90°
R-values:
R R work R free
0.185 0.183 0.222
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Roles of SAMHD1 in antiviral defense, autoimmunity and cancer.
Li et al. (2017)
6 more

6 mentions without citation

SAMHD1 Functions and Human Diseases.
Coggins et al. (2020)
5 more