› 4s0n

X-ray diffraction
1.5Å resolution

Crystal Structure of HLTF HIRAN Domain bound to DNA

Released:
DOI: 10.2210/pdb4s0n/pdb
PDB entry 4s0n coloured by chain, front view.
PDB entry 4s0n coloured by chain, side view.
PDB entry 4s0n coloured by chain, top view.
Source organisms:
Primary publication:
HLTF's Ancient HIRAN Domain Binds 3' DNA Ends to Drive Replication Fork Reversal.
Kile AC, Chavez DA, Bacal J, Eldirany S, Korzhnev DM, Bezsonova I, Eichman BF, Cimprich KA
Mol Cell 58 1090-100 (2015)
PMID: 26051180

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118157 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Helicase-like transcription factor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 130 amino acids
Theoretical weight: 14.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14527 (Residues: 55-180; Coverage: 13%)
Gene names: HIP116A, HLTF, RNF80, SMARCA3, SNF2L3, ZBU1
Sequence domains: HIRAN domain
5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3' Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 10 nucleotides
Theoretical weight: 3 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand DT 2 x DT
Ligand GOL 2 x GOL
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 61.026Å b: 74.211Å c: 66.18Å
α: 90° β: 113.68° γ: 90°
R-values:
R R work R free
0.154 0.153 0.185
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

48 review citations

DNA double-strand break repair-pathway choice in somatic mammalian cells.
Scully et al. (2019)
47 more

4 mentions without citation

Functions of SMARCAL1, ZRANB3, and HLTF in maintaining genome stability.
Poole et al. (2017)
3 more